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Fig. 2 Accumulation of DeltaD protein on the plasma membrane in the mib mutant. (A,B) Hindbrain expression of deltaD transcript in wild-type (WT) and mibta52b 10-somite stage (10 ss) zebrafish embryos. (C,D) DeltaD protein in areas corresponding to the rectangles in A and B. Embryos are stained with zdD2. The patches of DeltaD expression in D are in rhombomeres 3, 4 and 5. (E,F) deltaD transcript in wild type and mibta52b at the tail bud stage (tb) in hindbrain neurogenic domains. Area shown here corresponds approximately to rectangular areas in Fig. S1A,B (see supplementary material). mibta52b embryos do not have significantly exaggerated deltaD expression. (G,H) zdD2 staining in corresponding wild-type and mibta52b neurogenic domains. In mibta52b mutants, most DeltaD is at the cell surface, even when expression is not so exaggerated.