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Figure Caption
Fig. 3 Zebrafish igfbp-2b encodes a secreted protein that binds IGFs.
Conditioned media was prepared from HEK293T cells transfected with pCMV-human IGFBP-2-Myc (hIGFBP-2) or pCMV-zebrafish IGFBP-2b-Myc plasmids. The conditioned media were analyzed by Western immunoblotting (WB) using c-Myc antibody (left panel) and ligand blotting using DIG-labeled human IGF-1 (right panel).
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