ZFIN Image: Silvestri et al., 2008, Fig. 6

Image

Figure Caption

Fig. 6 Expression of Mutants Matriptase-2 in Zebrafish Affects Hemoglobinization of Erythrocytes

(A) Zebrafish embryos were injected with control (zcontrol MO) and matriptase-2 morpholino (zmt2 MO). Fourty-eight hours post injection, embryos were stained with o-dianisidine to detect hemoglobinized cells.

(B) Embryos as in (A) were homogenized, and RNA was extracted as described in Experimental Procedures. RT-PCR was performed using oligonucleotides specific for matriptase-2 (zmt2) and for the elongation factor 1α (zef1α). Four-hundred fifty embryos were injected, and the survival rate was 85%.

(C) Zebrafish embryos were injected with matriptase-2^wt (MT2^wt), MT2^R774C, and MT2^MASK expressing vectors, in the presence (+Fe) or not (-Fe) of iron dextran. At 48 hr after fertilization embryos were stained with o-dianisidine to detect hemoglobinized cells. Representative pictures of the injected fishes (200 for each construct, made in triplicate) are shown.

(D) MT2 levels were assayed by western blot analysis.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

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Reprinted from Cell Metabolism, 8(6), Silvestri, L., Pagani, A., Nai, A., De Domenico, I., Kaplan, J., and Camaschella, C., The Serine Protease Matriptase-2 (TMPRSS6) Inhibits Hepcidin Activation by Cleaving Membrane Hemojuvelin, 502-511, Copyright (2008) with permission from Elsevier. Full text @ Cell Metab.