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Fig. 5 The pigment pattern mutation Sparse, a kita null mutant, has decreased spontaneous GI contractions and an increased size of the GI tract. Optically transparent zebrafish larvae (7 dpf) were videotaped and contraction frequency was 0.59 ± 0.05 contractions/minute (mean ± SE, n = 20) in wild type, and 0.33 ± 0.03 contractions/minute (P < 0.05, n = 23) in homozygous Sparse mutant larvae. Cross-sectional area of the GI tract was measured in single frames for each experiment. Cross-sectional area was less for wild-type, 12,331 ± 467, compared to the Sparse mutant larvae, 14,985 ± 483 (P < 0.05).
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