ZFIN Image: Sakaguchi et al., 2008, Fig. S3

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Figure Caption

Fig. S3 The intrahepatic vascular and biliary networks in Vegf receptor inhibitor treated larvae. (A-F) Two examples (A-C and D-F) of Tg(flk1:EGFP)^s843 larvae treated with the Vegf receptor inhibitor SU5416 from 55 to 80 hpf. The larvae were visualized for GFP (green), Alcam (red) and Prox1 (blue) expression at 80 hpf. (A and D) Projected confocal images of Tg(flk1:EGFP)^s843 expression in the liver of a SU5416 treated larva in which the intrahepatic vascular network (schematically presented in A′ and D′) is disrupted compared to wild-type (Fig. 1A). (B and E) Projected confocal images of the liver visualized for Alcam expression at 80 hpf. The pattern of the Alcam positive network (schematically presented in B′ and E′) reflects the disrupted intrahepatic vascular network. (C and F) Merged images of Tg(flk1:EGFP)^s843 (green) and Alcam (red) expression shows that the disrupted intrahepatic networks still do not intersect.

Acknowledgments

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