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Fig. S2 Inhibition of Hedgehog signaling by cyclopamine treatment is reversible. (A-C) In situ hybridization detects ptc1 expression at the 10-somite stage. Lateral views, anterior towards the top. (A) Robust ptc1 expression is detected at the 10-somite stage in ethanol-treated wild-type embryos. (B) By contrast, ptc1 expression is undetectable in embryos exposed to CyA from the dome stage until the 10-somite stage. (C) Expression of ptc1 is evident in embryos that were exposed to CyA from dome stage until the six-somite stage; following washout at the six-somite stage, embryos were allowed to develop until the 10-somite stage.