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Fig. 2 Hedgehog signaling promotes the initial establishment of cardiomyocytes. (A-L) In situ hybridization detects expression of myocardial markers. Dorsal views, anterior towards the top. Scale bars: 100 μm (bar in D applies to D-I). MZsmo mutant and CyA-treated embryos exhibit reduced expression of (A-C) nkx2.5 at the seven-somite stage, (D-F) cmlc2 at the 18-somite stage, (G-I) vmhc at the 18-somite stage and (J-L) amhc at the 22-somite stage.
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