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Fig. 7 Knockdown of zdia2 inhibits cell protrusion formation of prechordal plate (primordial) cells and lateral (mesendodermal) cells.
(A) Embryos injected with the standard control MO (stdMO) or the zdia2 splice-blocking MO (sMO) were dechorionated, immobilized, monitored, and recorded on 10-minutes continuous time-lapse movies. (B) Protrusions formation of cells on focus in both stdMO- and zdia2 sMO-injected embryos were quantified separately by counting protrusions formed within the first 10 minutes form at least 3 different movies, respectively. Results were then analyzed and only upper error bars of standard deviations are shown. Different letters on top of each column indicate a significant difference between treatments (P<0.05).