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Fig. 2
Chronic exposure to ATR increases the percentage of female zebrafish.
A. The percentage of female zebrafish is shown after chronic exposure to DMSO and increasing ATR concentrations (μM). The number of female fish was assessed visually at three, four (data not shown) and at six months, as shown. Visual inspection for sex-specific landmarks (body shape, body color, fin shape) at both stages suggested an increase in the ratio of female to male fish. Following visual inspection of six month old fish, unambiguous assignment of sex was determined by morphological inspection of gonads. ATR treatment began at 17dpf post-hatching. B. Representative pictures of fish treated with ATR and corresponding gonadal sections stained with hematoxylin and eosin. Oocytes within the perinuclear follicles of the ovary are indicated (black arrowhead), and spermatozoa in the testis are indicated (white arrowhead).