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Fig. 1
Sequence and expression analyses of zebrafish k-ras.
(A) Zebrafish k-ras nucleotide and putative amino acid sequence. k-ras-MO1 and k-ras-MO2 binding sites are underlined. (B) Alignment of zebrafish K-ras with known Ras proteins of human, mouse and zebrafish. (C) Phylogenetic analysis of Ras proteins. (D) k-ras expression during embryonic development. k-ras transcripts were detectable from one cell stage (i) and then persist throughout the whole embryos (ii–vi). (i), one cell stage, side view; (ii), 3 hpf (hours-post fertilization), top view; (iii), 10 hpf, dorsal view, anterior to the top; (iv), 10 hpf, bottom view, dorsal to the top; (v), 20 hpf, lateral view, anterior to the left and dorsal to the top; (vi), 20 hpf, dorsal view, anterior to the left.