ZFIN Image: Gansner et al., 2008, Fig. 4

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Figure Caption

Fig. 4 pfd^gw1 mutants are sensitized to pharmacologic inhibition of lysyl oxidase. A-F: Clutches from pfd^gw1/+ intercrosses were incubated in vehicle (A,B), the copper chelator neocuproine (2 μM; C,D), or the lysyl oxidase inhibitor β-aminopropionitrile (1 mM; E,F). Notochord is normal in wild-type embryos treated with vehicle or neocuproine (A,C) and shows a very mild herniation event in β-aminopropionitrile (E, arrowhead). Notochords of pfd^gw1 mutants in neocuproine and β-aminopropionitrile (D,F, arrowheads) are substantially more distorted than mutants incubated in vehicle (B, arrowhead). Embryos were incubated in PTU to inhibit melanin pigmentation and photographed at 30 hours postfertilization (hpf).

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Acknowledgments

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