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Figure Caption
Fig. 1 A large portion of the yolk sac is absorbed during this sensitive developmental time window used to detect small molecule-mediated changes in fat metabolism (A). Extended incubation at 5 ng/mL nile red provides ideal signal to noise ratio for detection of anatomically localized fat stores (B). Overnight incubation was even better with the signal in the gall bladder often disappearing (shown in Fig. 4).
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Full text @ Nutr. Metab. (Lond).