ZFIN Image: Kapsimali et al., 2007, Fig. S29

Image

Figure Caption

Fig. S29 Mismatch test for let-7a, miR-92b, miR-153a and miR-181a.
To test the ability of LNA probes to discriminate between different members within a miRNA family, we performed in situ hybridization with single and double mismatch probes for let-7a, miR-92b, miR-153a and miR-181a. In parallel we performed in situ hybridization with the fully matching LNA probes. All probes were labeled and hybridized in parallel.

A,C,E,G: show in situ hybridization signal for full matching LNA probes miR-181a, let-7a, miR-92b and miR-153a, respectively.
B,D,F,H: show in situ hybridization signal for a double mismatch miR-181a probe (MMmiR-181a) and single mismatch probes let-7a, miR-92b and miR-153a (MMlet-7a, MMmiR-92b and MMmiR-153a), respectively.
Although there is a strong reduction in hybridization signal with the single mismatch probes (MMlet-7a, MMmiR-92b and MMmiR-153a), hybridization was only fully eliminated by the double mismatch probe MMmiR-181a. Thus, LNA probes may not be able to fully discriminate between miRNA family members that differ at a single position.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Open Access.

Full text @ Genome Biol.