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Fig. S1
Morpholino targeting of the TNF Receptor 1 (TR1) leads to complete splice blocking of transcript for 4 days.
Two splice blocking morpholinos targeting the exon-intron boundaries of E2I2 and E4I4 were used in combination to maximize disruption of transcript. Control embryos (Con) at 2 days (d) and 4d post fertilization in comparison with TR1 morphant transcripts (2d-4d) show splice blocking of native transcript during this time period.
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Reprinted from Immunity, 29(2), Clay, H., Volkman, H.E., and Ramakrishnan, L., Tumor necrosis factor signaling mediates resistance to mycobacteria by inhibiting bacterial growth and macrophage death, 283-294, Copyright (2008) with permission from Elsevier. Full text @ Immunity