|
Fig. S5
miR-34 expression in the zebrafish brain.
miR-34 shows very restricted expression in the larval brain with transcription restricted to ventral and lateral larval hindbrain areas, miR-34 conserves its expression in the adult hindbrain but additionally shows de novo expression in rostral and caudal nuclei, and thus changes dramatically its expression.
Specifically, at 3dpf and 5dpf, miR-34 is expressed in ventral and lateral medulla oblongata cells, (presumptive octaval area), locus coeruleus, trigeminal motor nuclei cells, Mauthner, and reticular formation cells (Table D). In the adult brain, miR-34 conserves its expression in respective nuclei and expands in specific nuclei and distinct cells of rostral brain, including epithalamic, pretectal, posterior tuberculum, tectal and tegmental cells, as well as in areas of dorsal hindbrain (cerebellar granular layer, facial and vagal lobes, Table I).
A. transverse section through the larval rostral hindbrain at the level of the octaval ganglion (OG) showing miR-34 expressing cells in the ventral medulla oblongata (MO), superior reticular formation (SRF), likely the trigeminal motor nucleus (NV?) and octaval ganglion (OG).
B. transverse section through the larval hindbrain at the level of the otic capsule (ot, caudal to section A) showing miR-34 expressing cells in the ventrolateral medulla oblongata (MO, likely octaval area, OA) and intermediate reticular formation (IMRF).
C. transverse section through the caudal larval hindbrain at the level of the inferior olive (IO) showing miR-34 expressing cells in the ventrolateral medulla oblongata (MO, arrowhead).
D. transverse section through the larval hindbrain at the level of the octaval ganglion (OG) showing miR-34 expressing cells in the ventrolateral medulla oblongata (MO, likely octaval area, OA), intermediate reticular formation (IMRF), Mauthner neuron (MAC) and octaval ganglion (OG).
E. transverse section through the larval hindbrain at the level of the octaval ganglion (OG, section caudal to section D) showing miR-34 expressing cells in the ventrolateral medulla oblongata (MO), likely octaval area (OA), intermediate reticular formation (IMRF) and octaval ganglion (OG). The arrowhead points at miR-34 expressing cells in the ventral hindbrain in proximity to the octaval nerve (VIII).
F. transverse section through the caudal larval hindbrain at the level of the inferior olive (IO) showing miR-34 expressing cells (arrowhead) in the ventrolateral medulla oblongata (MO), likely ventral to the vagal motor nucleus (NX).
G. transverse section through the adult epithalamus showing miR-34 expressing cells (arrowhead) in the dorsal habenular nucleus (Had).
H. transverse section through the adult hypothalamus showing miR-34 expressing cells in the nucleus of the paraventricular organ (nPVO).
I. transverse section through the adult cerebellum showing miR-34 expressing cells in the cerebellar granular layer (CeGL).
J. transverse section through the adult rostral hindbrain showing miR-34 expressing cells in the locus coeruleus (LC) and superior reticular formation (SRF).
K. transverse section through the adult hindbrain at the level of the trigeminal root showing miR-34 expressing cells in the primary sensory trigeminal nucleus (NVs), dorsal part of trigeminal motor nucleus (NVmd) and superior reticular formation (SRF).
L. transverse section through the adult lateral hindbrain at the level of the Mauhtner neuron (MAC) showing miR-34 expressing cells in the MAC, magnocellular (MaON), anterior (AON) octaval and medial octavolateral (MON) nuclei.
M. transverse section through the adult superficial pretectum showing miR-34 expressing cells in the magnocellular superficial pretectal nucleus (PSm) and the optic tectum (TeO).
N. transverse section through the adult pretectum and optic tectum showing miR-34 expressing cells in the tectal periventricular grey (pgz) and central (arrowhead) zones and accessory pretectal nucleus (APN).
O. transverse section through the adult lateral hindbrain at the level of the octaval nerve (VIII, caudal to section L) showing miR-34 expressing cells in the descending octaval (DON) and medial octavolateral (MON) areas and the intermediate reticular formation (IMRF). The arrowhead points at miR-34 expressing cells in the ventral hindbrain in proximity to the octaval nerve (VIII).
P. transverse section through the adult lateral hindbrain at the level of the vagal nerve (X), showing miR-34 expressing cells in the facial lobe (LVII), posterior octaval area (PON) and lateral to the medial longitudinal fascicle (MLF).