ZFIN Image: Wang et al., 2008, Fig. 1

Image

Figure Caption

Fig. 1 Screen for Tg(zlmo2:Cre) F0 founders. A: Schematic representation of the lmo2-Cre construct. The Cre gene was fused to a 2.5-kb lmo2 promoter. pA denotes SV40 poly A element. Arrows denote the position of primers used for genomic PCR screening. B: The representative results of genomic PCR screen for Tg(zlmo2:Cre) F0 founder pairs. C: The actin-LDL-EGFP reporter plasmid used to test the Cre activity in the embryos of the Tg(zlmo2:Cre) F0 founder. The Cre activation will result in the excision of DsRed with the simultaneous initiation of EGFP expression. D: Red (top) and green (middle) fluorescence observed in embryo injected with the reporter plasmid at 36 hpf. Circulating blood cells expressing both DsRed and EGFP can also be seen (arrows). Embryos are lateral views with heads to the left.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.