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Fig. S6
RFP expression analysis at 20 hpf for k-ras-5′UTR-RFP injected embryos, indicating the targeting specificity of k-ras morpholino antisense oligo.
(A) Embryo injected with k-ras-5′UTR-RFP/PCS (red fluorescent protein reporter was down stream of K-ras 5′UTR and was cloned into PCS2 vector) construct, showing strong RFP signal.
(B) Embryo co-injected with k-ras-5′UTR-RFP/PCS and k-ras-MO1, showing very weak RFP signal, indicating the blockage of RFP protein expression by k-ras-MO1.
(C) Embryos from different treatments, showing the different RFP strength under the same exposure. These embryos were (i), injected with k-ras-5′UTR-RFP/PCS alone; (ii), co-injected with k-ras-5′UTR-RFP/PCS and k-ras-MO1; and (iii), wild type embryo with no injection.