ZFIN Image: Kim et al., 2008, Fig. 1

Image

Figure Caption

Fig. 1 gfap⁺ cells are radial glia in embryonic and postembryonic zebrafish spinal cord. All images are transverse sections of spinal cords, dorsal to top. A,B: Spinal cords of 36 hours postfertilization (hpf) Tg(gfap:GFP) embryos labeled by gfap (A) and egfp (B) RNA in situ hybridization. C: gfap RNA expression at 36 hpf. D: Glial fibrillary acidic protein (GFAP) expression detected by immunocytochemistry at 48 hpf. Brackets in A-D mark ventral spinal cord cells that do not express the endogenous gfap gene or the reporter transgene. Arrowheads mark floor plate. E-G: Anti-Zrf-1 antibody labeling (red) and enhanced green fluorescent protein (EGFP) fluorescence (green) of Tg(gfap:GFP) animals at 3 days postfertilization (dpf), 7 dpf, and 3 months. Numerous Zrf-1⁺ EGFP⁺ radial glia appear as yellow fibers. Arrows indicate Zrf-1⁺ GFAP^- radial glial cells in ventral most spinal cord. H: Anti-brain-lipid-binding protein (BLBP) antibody (red) labeling of 3-month-old adult Tg(gfap:GFP) fish. BLBP⁺ EGFP⁺ radial glia appear yellow. Arrows indicate BLBP⁺ EGFP^- radial fibers in ventral spinal cord. Scale bar = 20 μM in A-F, 80 μM in G,H.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.