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Fig. S3 Phosphoinositide 3-kinase (PI3K) signaling does not regulate the movements of endodermal cells. (A-F) Confocal images of endodermal cells in Tg(sox17:EGFP) transgenic embryos at the three-somite stage. Dorsal views, anterior to the top. One marginal blastomere cell at the 128-cell stage has been transformed into an endodermal fate by the misexpression of cas mRNA (2 pg). The injected cell was labeled with dextran-Alexa594. (A-C) The progeny of the injected cell intermingle with EGFP-expressing endodermal cells. (D-F) The migration of cells co-injected with both cas (2 pg) and a dominant-negative form of phosphoinositide 3-kinase (dnPI3K) (4 pg) mRNAs was not changed relative to cells injected with cas mRNA alone.