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Fig. S2 The 10E4 epitope is not expressed in the developing cartilage. Wildtype (A,C,E) and pic-/- (B,D,F) localisation of the 10E4 epitope (in green) at 60hpf. Cartilage (collagen type II) is shown in red and nuclei in blue (C–F). Side views of the whole fish (A,B), ventral views of the head (C,D) and high magnification pictures of the ceratohyal (E,F). The 10E4 epitope is detected predominantly on basal laminae but is undetectable in the developing cartilage at the time of chondrocyte stacking. Panel D scale bar = 100μM. Panel F scale bar = 10μM.
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