ZFIN Image: Martin et al., 2008, Fig. 2

Image

Figure Caption

Fig. 2 Bra Knockdown Enhances the ntl Phenotype

(A) A schematic of Bra knockdown strategies. MO1 targets the bra splice acceptor site at the intron 1-exon 2 junction. Primers in the first and third exons were used to analyze the bra transcript in uninjected (UC) and MO1-injected embryos. The improperly spliced bra transcript results in a truncated protein that lacks the T box. MO2 targets the translation start site of bra.

(B–E) Expression of myoD at 24 hpf in an (B) uninjected +/+ or +/- sibling, (C) uninjected ntl mutant, (D) MO1-injected ntl mutant, and (E) MO2-injected ntl mutant.

(F–I) Expression of tbx6 in four-somite embryos.

(J–Q) Expression of ntl at 75% epiboly and bud. Arrows and arrowheads show diminished notochord expression.

Embryos are shown from a (B-E) lateral view with anterior to the left, (F-I) posterior view with dorsal to the top, (J-M) dorsal view with animal pole to the top, and (N-Q) dorsal view with anterior to the left.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

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Reprinted from Developmental Cell, 15(1), Martin, B.L., and Kimelman, D., Regulation of canonical Wnt signaling by Brachyury is essential for posterior mesoderm formation, 121-133, Copyright (2008) with permission from Elsevier. Full text @ Dev. Cell