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Fig. S1 Spatio-temporal distribution of global ERK protein, compared to active dual-phosphorylated ERK (dpERK) protein by immuno-histochemistry. DpERK signal was stronger on the cleavage-sites (2-cell and 16-cell stage). During gastrulation dpERK signal was enhanced in the margin (►). After completion of gastrulation stronger signals were observed in the tailbud, MHB (*) and the anterior neural boundary (●). (5x objective, Biorad confocal laser scanning microscope).
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Reprinted from Developmental Biology, 319(2), Krens, S.F., He, S., Lamers, G.E., Meijer, A.H., Bakkers, J., Schmidt, T., Spaink, H.P., and Snaar-Jagalska, B.E., Distinct functions for ERK1 and ERK2 in cell migration processes during zebrafish gastrulation, 370-383, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.