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Fig. 1 Molecular characterization and expression pattern of zebrafish C1q-like. (A) Amino-acid alignment of C1q-like from zebrafish with putative C1q-like factor from Carassius auratus (Ca), brook trout (Salvelinus fontinalis, Sf), rainbow trout (Oncorhynchus mykiss, Om), Norway rat (Rattus norvegicus, Rn) and rhesus monkey (Macaca mulatta, Mm). Similar and identical amino acids are highlighted in grey and black boxes. And the percentages of identities and similarities were shown compared zebrafish C1q-like with other C1q-like factors. (B, C) Expression of C1q-like in adult tissues (B) and embryonic development stages (C) evaluated by real-time PCR and normalized to beta-actin mRNA. The data represent mean ± SD of three separate experiments. (D) Expression pattern of zebrafish C1q-like. Whole-mount RNA in situ hybridization and immunohistochemistry were performed using a C1q-like specific antisense riboprobe (a, c, e, g) and anti-C1q-like antibody (b, d, f, h) on embryos at the indicated stages. The arrows indicated the signals in the anterior and head. Embryos in panels a and b are lateral views with the animal pole toward the top. The embryos in other panels are lateral views, with dorsal toward the top and anterior to the left.
Reprinted from Developmental Biology, 319(2), Mei, J., Zhang, Q.Y., Li, Z., Lin, S., and Gui, J.F., C1q-like inhibits p53-mediated apoptosis and controls normal hematopoiesis during zebrafish embryogenesis, 273-284, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.