Image
|
Figure Caption
Fig. 8 atp6v0b morpholinos do not phenocopy blowout. (A) The atp6v0b predicted genomic organization. atp6v0b MO targets the exon1/intron1 splice junction and leads to the inclusion of a 705 bp intron1 and the introduction of a premature stop codon. (B) RT-PCR validation of splice blocking efficacy of the atp6v0b MO. (C) Control injected and (D) atp6v0b morphant (13 ng) at 5 dpf. Morphants display oculocutaneous albinism and retinal degeneration but show no signs of colobomas even at the highest doses of MO injection.
Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Reprinted from Developmental Biology, 319(1), Lee, J., Willer, J.R., Willer, G.B., Smith, K., Gregg, R.G., and Gross, J.M., Zebrafish blowout provides genetic evidence for Patched1-mediated negative regulation of Hedgehog signaling within the proximal optic vesicle of the vertebrate eye, 10-22, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.