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Fig. 1 blowout mutants present with colobomas. Wild-type (A, C) and blw (B, D) mutant embryos at 50 hpf (A, B) and 5 dpf (C, D) imaged laterally (A, B) and ventrally (C, D). Colobomas can be unilateral or bilateral, and these blw mutants show bilateral incidence. Arrows in panels A and B point to the choroid fissure. Retinal tissue expelled from each eye has been outlined to illustrate the extent of the coloboma in panels C and D. Transverse histological sectioning of wild-type (E, G) and blw (F, H, I) eyes. Colobomas are obvious at 3 dpf in blw (F); however, at later time points their severity varies ranging from mild (H) to severe (I). Lens and retinal development appear normal in blw mutants. Anterior is up in panels A–D. Dorsal is up in panels E–I. Scale bars are 100 μm.
Reprinted from Developmental Biology, 319(1), Lee, J., Willer, J.R., Willer, G.B., Smith, K., Gregg, R.G., and Gross, J.M., Zebrafish blowout provides genetic evidence for Patched1-mediated negative regulation of Hedgehog signaling within the proximal optic vesicle of the vertebrate eye, 10-22, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.