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Fig. S2 (A-F) Confocal sections of Tg(AANAT2:GFP) (A-C) and Tg(HuC:GFP) (D-F) epiphysis stained with a caspase 3 antibody and GFP in wild-type (A), mib mutant (B) and DAPT-treated embryos (C) at 48 hours of development. The number of apoptotic cells is increased in mib mutant compared with wild-type embryos and in DAPT-treated embryos compared with mib mutants. (G) Counts of caspase 3+ cells, Tg(HuC:GFP)+/caspase 3+ and Tg(AANAT2:GFP)+/caspase 3+ cells. Numbers indicate average±s.d. *P<0.05; **P<0.001; ***P<0.0005 using a t-test. nd, not determined.
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