ZFIN Image: Rebagliati et al., 1998, Fig. 5

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Figure Caption

Fig. 5 Rescue of the cyc^b16 phenotype by injection of a cyc expression construct. Wild-type (A, D, and G), cyc (B, E, and H), and cyc rescued by injection of 0.5 pg DNA per embryo (C, F, I, and K) are shown, as hybridized with twhh (A-F and K) or shh and pax2 (G-I). (J) After staining, the embryos were genotyped by PCR with primers that span an intron in the cyc gene; injected embryos also showed amplification product from the intronless expression plasmid. Aliquots of the same DNA samples also were amplified with primers for the unlinked ndr1 gene as a positive control. Lanes: 1, amplification of DNA from embryo in C; 2, F; 3, I; 4, injected wild type; 5, uninjected wild type; 6, uninjected cyc.

Acknowledgments

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