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Fig. 1 (A) ndr2 RNA is present in wild-type (WT) but not homozygous cycb16 embryos, as seen by in situ hybridization at the shield stage (28). (B) ndr2 is deleted in cycb16 DNA, visualized by PCR. Lanes: 1, ndr2 primer pair 1 + ndr2 plasmid; 2, ndr2 primer pair 1 + wild-type genomic DNA; 3, ndr2 primer pair 1 + cycb16 homozygous genomic DNA; 4, ndr2 primer pair 2 + ndr2 plasmid; 5, ndr1 primers + ndr2 primer pair 2 + cycb16 genomic DNA; 6, ndr1 primers + ndr1 plasmid.
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Full text @ Proc. Natl. Acad. Sci. USA