ZFIN Image: Schenck et al., 2001, Fig. 7

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Figure Caption

Fig. 7 The FMRP sequence encoded by FMR1 exon 7 is implicated in the interaction with CYFIP1. Yeast two-hybrid assay between CYFIP1 and chimeric FMRP/FXR1P and FMRP/FXR2P proteins. FMRP sequences are in red, FXR1P in yellow, and FXR2P in blue. In hybrid constructs 1.1–2.2, increasing parts of FMRP exon 7 were replaced by FXR1P or -2P, in hybrids 3.1 and 3.2, the entire exon 7. In construct 4.1, exon 4-7/8 of FMR1 is substituted by the FXR1 sequence. On the right of each construct, the strength of interaction with CYFIP1 in relation to interaction FMRP--CYFIP1 is indicated. For constructs 1.1, 1.2, 3.1, and 3.2, a 5-bromo-4-chloro-3-indolyl β-D-galactoside filter lift assay is shown.

Acknowledgments

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