ZFIN Image: Schenck et al., 2001, Fig. 6

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Figure Caption

Fig. 6 The FMRP sequence encoded by FMR1 exon 7 is implicated in the interaction with CYFIP1. Yeast two-hybrid assay between CYFIP1 and chimeric FMRP/FXR1P and FMRP/FXR2P proteins. In hybrid constructs, 1.1-2.2 increasing parts of FMRP exon 7 were replaced by FXR1P/2P, in hybrids 3.1 and 3.2 the entire exon 7. In construct 4.1, exon 4-7/8 of FMR1 is substituted by the FXR1 sequence. To the right of each construct, the strength of interaction with CYFIP1 in relation to interaction FMRP-CYFIP1 is indicated. For constructs 1.1, 1.2, 3.1, and 3.2, also an X-gal filter lift assay is shown. A more detailed representation of the hybrid constructs, the FMRP exon 7 region, and the corresponding FXR1P/2P sequences is published as supplemental data on the PNAS web site, www.pnas.org.

Acknowledgments

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