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Figure Caption
Fig. 4 Localization of epitope-tagged Brd4 protein. RNA encoding HA- or FLAG-tagged zebrafish brd4 was injected into one- to two-cell stage embryos and visualized by antibody staining. Each column represents a separate embryo at the stage indicated. A-A″,B-B″: FLAG-Brd4. C-C″,D-D″: HA-Brd4. A-C: Pre-MBT (512 cell stage) embryos with mitotic chromosomes (A, anaphase and C, prophase), and interphase nuclei (B) shown. D: Post-midblastula transition (MBT, sphere to dome stage) embryo presents both mitotic chromosomes and interphase nuclei in one embryo. Scale bar = 10 μm.
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