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Fig. S3 PDX1 binds to PC3b and not to PC3a. EMSAs were performed on PC3 probes of pax6a (PC3a) and pax6b (PC3b) using nuclear extract of pancreatic TU6 cells. 10 ng of unlabeled oligonucleotides PC3a (lanes 2, 6), and PC3b (lanes 3, 7) were added as competitors. Abbreviations: PBX1a and PREP1, recombinant PBX1a and PREP1 proteins produced in vitro in a reticulocyte extract; PDX1 rec, PDX1 recombinant.
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Full text @ BMC Dev. Biol.