ZFIN Image: Ciruna et al., 2002, Fig. 1

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Figure Caption

Fig. 1 Germ-line replacement strategy using a GFP-nos1-3′UTR labeling technique. (A) Overview of transplantation strategy showing the transfer of cells from the margin of GFP-nos1-3′UTR-labeled mutant donor embryos into the margin of αPGC-MO-injected WT hosts. "m" refers to any zygotic mutation, in this case mil^te273. (B) At 30 hpf, PGCs from injected control mil_ix embryos are specifically labeled with GFP (arrowhead). (C) Host embryos were screened at 30 hpf for the presence of GFP-labeled donor PGCs and their normal migration into the gonadal mesoderm at the anterior region of the yolk extension (arrow).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA