Fig. 3 In situ hybridization demonstrates the expression of chordin (a–d), goosecoid (e–h), and Vent (i and j) in embryos harvested 8 h postinjection with control mRNA synthesized from PCS2 vector control or gelsolin mRNA, as indicated. Control (a and e), gelsolin MO-injected (1 ng/E; b and f), gelsolin MO-injected (2.5 ng/E; c and g), and gelsolin mRNA-injected (50 pg/E; d and h) embryos are shown, with the anterior side to the right. Vent mRNA expression is shown in embryos injected with control MO (i) and gelsolin MO (j) at 2.5 ng/E. The animal pole of the embryos is oriented upward. (k) A schematic of how gelsolin might regulate cell fates in zebrafish.
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