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Fig. 3 Semiquantitative RT-PCR analysis of FACS-sorted blood cell populations in the kidney (K) and thymus (T) of lck-GFP and rag2-GFP transgenic fish. GFP-positive (+) and -negative (-) blood cell populations are shown. Results for "No RT Control" show absence of genomic DNA contamination in samples. The β-actin PCR control was completed on genomic DNA. Because the β-actin primers span an intron, PCR amplifies a 100-bp larger fragment than seen in RT samples.
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Full text @ Proc. Natl. Acad. Sci. USA