ZFIN Image: Kawahara et al., 2005, Fig. 3

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Figure Caption

Fig. 3 Effect of gadd45β MOs on somite segmentation. MOs (control-MO, 15 ng; GADD45β1-MO + GADD45β2-MO, 7.5 ng each) were injected into one- or two-cell-stage embryos. Dorsal view, anterior is up (A–C and J–L). Posterior view, dorsal is up (D–I). Lateral view, anterior is left (M–O). (A–C) Live embryos at 8- or 10-somite stages. Injection of GADD45β1-MO plus GADD45β2-MO, but not control-MO, caused severe defects in somite segmentation (A–C). (D–O) Whole-mount in situ hybridization with probes are shown at the upper right of each micrograph. Expression of mesp-a and her1 in the anterior PSM and segmental expression of MyoD and fgf8 (red asterisk) in the somites were disorganized in the GADD45β1-MO + GADD45β2-MO-injected embryos (D–O). Expression of fgf8 in the PSM (red bracket) was expanded in the double GADD45β MO-injected embryos (M–O).

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Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA