Fig. 7

Fig. 7 mab-9 mutants have motor neuron axon guidance defects. (A) WT adult hermaphrodite carrying an unc-25::GFP reporter specific for DD/VD GABAergic neurons). Motor neuron cell bodies are located in the ventral nerve cord. Commissures extend around the circumference of the worm, 17 of which run on the right hand side with 2 running on the left hand side. As the commissures reach the dorsal side they bifurcate to form the dorsal nerve cord (out of focal plane). (B and C) Adult mab-9(e2410) animals carrying the unc-25::GFP reporter. DD/VD motor neurons extend circumferential commissures that are misguided. (B) Misguided commissure stalling at the ventral side of dorsal muscle (arrow). (C) Misguided commissure stalling at the lateral nerve cord (arrow). Misguided axons often cross a number of adjacent commissures. (D) WT adult hermaphrodite carrying an unc-53::GFP reporter, expressed in DA and AS motor neurons. Again, commissures can be seen to extend around the circumference of the worm. (E) Adult mab-9(e2410) adult carrying the unc-53::GFP reporter. Misguided DA/AS motor neurons are visible (arrow). Dorsal is up, posterior to the right in all panels.