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Fig. 9 Overexpression of admp, tr-admp, or admp-morpholino in din mutants. tr-admp RNA (600 pg), admp RNA (600 pg), or admp-morpholino (Mo) (250 μM) was injected at the one-cell stage into the progeny of a din-/- x din-/+ cross. (A–D) Phenotypes were observed live after 1 day of development or (E–I) after in situ hybridization at the 10-somite stage; fkd6, her5, and gata1 in dark blue, hgg1, krox20, and ntl in red. (A) Uninjected embryo, din-/- phenotype. (B) tr-admp-injected din-/- embryo, partially rescued. (C) admp injected din-/- embryo, enhanced phenotype. (D) Mo-injected din-/- embryo, partially rescued. (A–D) Arrowhead, compare the size of the eye; arrows, ventral tail fin and vein, lost in (B); and amount of blood cells slightly reduced in (B) and (D). (E) Uninjected, -/+ or +/+ embryo. (F) Uninjected embryo, din-/- phenotype. gata1 expression domain is enlarged (arrowhead) (G) admp injected din-/- embryo, enhanced phenotype. Note the absence of posterior notochord (arrow) and the enlarged gata1 expression domain (arrowhead). (H) Mo-injected din-/- embryo, partially rescued. The notochord is enlarged (arrow); compare with (A) and (B). (I) tr-admp-injected din-/- embryo, partially rescued. The notochord is enlarged (arrow).
Reprinted from Developmental Biology, 241(1), Willot, V., Mathieu, J., Lu, Y., Schmid, B., Sidi, S., Yan, Y.-L., Postlethwait, J.H., Mullins, M., Rosa, F., and Peyriéras, N., Cooperative action of ADMP- and BMP-mediated pathways in regulating cell fates in the zebrafish gastrula, 59-78, Copyright (2002) with permission from Elsevier. Full text @ Dev. Biol.