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Fig. 7 desb420 mutants have excess primary motoneurons. CaP and VaP motoneurons (arrowheads) as revealed by islet2 RNA in situ hybridization at 20 h in a wild-type (A) and mutant (B) embryo. (C) Counts of islet1 and islet2 expressing cells at 20 h in spinal hemisegments 5–9; n = 10 embryos for each point. For these and all subsequent histograms the mean ± 95% confidence interval is plotted. Significance was determined by Student’s t test with *, P = 0.01–0.001; **, P < 0.001. Scale bar, 25 μm.
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Reprinted from Developmental Biology, 237(2), Gray, M., Moens, C.B., Amacher, S.L., Eisen, J.S., and Beattie, C.E., Zebrafish deadly seven functions in neurogenesis, 306-323, Copyright (2001) with permission from Elsevier. Full text @ Dev. Biol.