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Fig. 5 Pancreatic specific expression of the zebrafish nkx2.2, islet-1, and pax6.2 mRNAs. Whole-mount in situ hybridization of wild-type zebrafish embryos at the (A, A′) 10-somite stage; (C, C′, E, E′) 12-somite stage, and (B, D, F) 24 hpf performed with (A, A′, B) nkx2.2, (C, C′, D) islet-1, and (E, E′, F) pax6.2 antisense mRNA probes. Lateral views, dorsal to the right (A, A′, C, C′, E, E′) or to the top (B, D, F). Pancreas-specific expression domain are indicated (arrowheads).
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Reprinted from Developmental Biology, 230(2), Biemar, F., Argenton, F., Schmidtke, R., Epperlein, S., Peers, B., and Driever, W., Pancreas development in zebrafish: early dispersed appearance of endocrine hormone expressing cells and their convergence to form the definitive islet, 189-203, Copyright (2001) with permission from Elsevier. Full text @ Dev. Biol.