|
Fig. S1 Syn4 is required for the development of NC derivatives. (A-K) Lateral (A,B,D,E,F,H,I,J) and ventral (C,G,K) views of zebrafish embryos showing the expression of GFP in the cartilage of the transgenic line sox10:egfp (B,C,F,G,J,K) and the development of melanocytes (D,H,L). (A-D) Embryos injected with control MO. (E-H) Embryos injected with 6 ng of syn4 MO2. (I-K) Embryos injected with 6 ng of syn4 MO1. Note that both syn4 MOs produced an equivalent inhibition in cartilage development (45% inhibition for MO2, n=60; 52% inhibition for MO1, n=104). (D,H,L) syn4 MO2 produced a clear inhibition in melanocyte development, similar to the one produced by syn4 MO1 (see Fig. 2F,I).