Fig. 10

Fig. 10 hdac3 mRNA partially rescued the liver defects in VPA-treated embryos while hdac1 mRNA could not. 5′-capped mRNAs of hdac1 and hdac3 were synthesized in vitro and used to rescue the liver defects in VPA-treated embryos in Tg (lfabp:RFP, elaA:EGFP) by microinjection. Overexpression of hdac1 mRNA (0.3 ng/embryo) did not generate any obvious impact on liver development (C vs. A) and hdac1 mRNA failed to rescue the liver defects in VPA-treated embryos (D vs. B). In contrast, hdac3 mRNA (0.3 ng/embryo) led to a slight increase in liver size compared to control (E vs. A). It also readily rescued the small liver defects in VPA-treated embryos although not to the same size of the control at the doses analyzed (F vs. B). Injection of both hdac1 and hdac3 mRNA together (0.3 ng/embryo each) rescued the liver defect under VPA to similar extent as hdac3 mRNA alone (H vs. B). All images are lateral view of 5 dpf embryos, anterior toward the left. Scale bar is 100 μm.