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Fig. S2
Genotyping of sri mutant and wild type larvae
DdeI restriction digest of fragment spanning the sri mutation site, showing the genotyping of fish from sri/+ x sri/+ parental cross. Genotyping was carried out on the 5 dpf larvae that were analysed for the pancreatic phenotype in Figure 1 of the paper.
The ratio of WT : heterozygous : homozygous mutant genotypes did not deviate significantly from the expected frequency of 1:2:1, with a Chi square (Σ2) value of 1.390, showing that the deviation observed is not significant, with a p value of 0.499. (observed ratios 7:23:11 at 5 dpf)
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