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Fig. S3
Expression of NS- and LS-Shp2 Did Not Induce Defects in Cell Specification
Synthetic RNA encoding NS-Shp2 (D61G or T73I) or LS-Shp2 (A462T or G465A) was injected into one-cell stage zebrafish embryos. The embryos were fixed at 6 hpf and in situ hybridization was done with the indicated probes. Animal pole views are depicted here. Expression of the mesendodermal marker ntl and of the organiser marker gsc remained unchanged in the NS- and LS-Shp2–expressing embryos.
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