Fig. 7

Fig. 7 foxA2 is required for floor plate formation. Left panels show expression of GFP (A), netrin1 (C, E), shh (G, I), and collagen2a1 (K) in uninjected control embryos. Black arrow, expression in the floor plate (GFP, netrin1, shh, col2a1); black arrowhead, expression in the hypochord (GFP, netrin1, col2a1); and red arrowhead, expression in the notochord (shh, col2a1). Right panels represent expression of the same markers in foxA2 morpholino-injected embryos. Note that expression of GFP (B), netrin1 (D, F), shh (H, J), and collagen2a1 (L) is diminished or totally absent (blue arrowhead). Expression of these markers is normal in the notochord (red arrowhead: shh, col2a1), hypochord (black arrowhead: GFP, netrin1, col2a1), and ventral brain (shh, netrin1). (M) In FoxA2 knockdown embryos, the characteristic columnar shape of floor plate cells seems to be maintained (M, green arrowhead). These cells do not express col2a1 even in strongly overstained embryos (M). Abbreviations: CO, control; MO, foxA2 morpholino-injected embryo. Scale bar in (L) represents 100 μm.