ZFIN Image: Kawahara et al., 2002, Fig. 1

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Figure Caption

Fig. 1 Molecular analysis of mbx. (A) Predicted protein sequence of Mbx. Identical amino acids between zebrafish (z) and human (h) Mbx are boxed, and the homeodomain is indicated by red letters. Asterisks show the five amino acids specific to the long form. (B) Sequence alignment of homeodomains of Mbx with Alx4 (Qu et al., 1997), Cart1 (Zhao et al., 1993), Pax7 (Jostes et al., 1990), and Ptx2 (Arakawa et al., 1998); hyphens indicate identity. (C) Phylogenetic tree, based on full-length amino acid sequences, including mouse Mbx (Miyamoto et al., 2002). (D) RT-PCR analysis of mbx-S and mbx-L expression in zebrafish at the indicated stages. pBS-mbx-S and pBS-mbx-L are positive control plasmids for the short and long splicing form, respectively. In the upper panel, common primers for mbx-S and mbx-L genes were used, while long form-specific primers were used in the second panel. The third panel represents EF1α loading controls.

Acknowledgments

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Reprinted from Developmental Biology, 248(1), Kawahara, A., Chien, C.-B., and Dawid, I.B., The homeobox gene mbx is involved in eye and tectum development, 107-117, Copyright (2002) with permission from Elsevier. Full text @ Dev. Biol.