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Fig. 6 ngn1 morpholino oligonucleotides block differentiation of all cranial ganglia. Control embryos are shown in (A, C, E); ngn1 morpholino injected embryos in (B, D, F). (A, B) Lateral flat mounts of embryos expressing neuroD at 36 h. After ngn1 morpholino injection, neuroD expression is abolished in cranial ganglia (arrows) but not in other regions. (C, D) Lateral view of Hu expression in cranial ganglia at 96 h. In most cases, cranial ganglia are absent, but in some cases, a few neurons remain (arrows). (E, F) Lateral view of Hu expression in the zebrafish trunk at 96 h. The dorsal root ganglia (arrows) are eliminated by ngn1 morpholino injection, while enteric neurons are unaffected. ad, anterodorsal lateral line ganglion; av, anteroventral lateral line ganglion; e, enteric neurons; f, facial ganglion; g, glossopharyngeal ganglion; m, middle lateral line ganglion; o, octaval/statoacoustic ganglion; p, posterior lateral line ganglion; v, vagal ganglia. Bar, 100 μm (A, B) and (E, F); 30 μm (C, D).
Reprinted from Developmental Biology, 251(1), Andermann, P., Ungos, J., and Raible, D.W., Neurogenin1 defines zebrafish cranial sensory Ganglia precursors, 45-58, Copyright (2002) with permission from Elsevier. Full text @ Dev. Biol.