ZFIN Image: Chung et al., 2008, Fig. 6

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Figure Caption

Fig. 6 Cell-Cell Interactions between Endodermal Cells Are Required for Dorsal Pancreatic β Cell Induction

(A) Schematic diagram of the cell transplantation protocol. cas-overexpressing wild-type Tg(ins:GFP) donor cells were transplanted into wild-type or smo mutant Tg(ins:GFP) hosts.

(B and C) Dorsal views of wild-type (B) and smo mutant (C) hosts with the endodermal cells derived from wild-type donor cells labeled with rhodamine dextran (red) at 18 hpf. (B) Wild-type hosts show wild-type-like Tg(ins:GFP) expression after transplantation. (C) Tg(ins:GFP) expression (arrows) is restored in smo mutant hosts when wild-type cells contribute to the endoderm (44.5%, n = 48/108).

(D and E) Transverse confocal images of smo mutant hosts stained for GFP (green) and β-catenin (white) at 18 hpf. Wild-type donor cells are labeled with rhodamine dextran (red). At this stage, parts of the endodermal tissue have become multilayered as cells are converging actively to the midline. (D) Most of the Tg(ins:GFP)-expressing cells in smo mutant hosts are derived from wild-type donor cells (arrows). (E) However, in several embryos (n = 6/48), lateral endoderm derived from wild-type donor cells correlates with Tg(ins:GFP) expression in medially located smo mutant host endoderm (arrow).

(F) Schematic diagram of the cell transplantation protocol. cas-overexpressing wild-type donor cells were transplanted into wild-type or smo mutant Tg(ins:GFP) hosts.

(G and H) Lateral views of live smo mutant hosts for Tg(ins:GFP) (green) and rhodamine dextran (red) at 30 hpf. (G) When wild-type donor cells were not incorporated into the endoderm, smo mutants showed a complete absence of Tg(ins:GFP) expression in most cases (48/50) or at most 2 Tg(ins:GFP)-expressing cells (2/50). (H) When wild-type donor cells were incorporated into the endoderm, several experimental smo mutants (n = 7/26) showed 4–7 rescued Tg(ins:GFP)-expressing cells (arrows; 5 rescued cells in this figure). (In these experimental smo mutants, pancreatic β cells likely comprise both wild-type and smo mutant cells.)

Acknowledgments

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Reprinted from Developmental Cell, 14(4), Chung, W.S., and Stainier, D.Y., Intra-endodermal interactions are required for pancreatic beta cell induction, 582-593, Copyright (2008) with permission from Elsevier. Full text @ Dev. Cell