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Fig. 4 Expression of raldh4 during early development of zebrafish. (A) RT-PCR analysis showing the expression of raldh4 gene. (2h–7d) Lanes using as templates total RNA isolated from 2 hpf–7 dpf embryos, respectively; +, positive control lane using raldh4 cDNA as template; -, negative control lane using water as template; PCR results give a 513 bp amplified fragment from raldh4 cDNA. Amplification of a 716 bp β-actin cDNA fragment is a control of RT-PCR sensitivity in the assay. (B-F,H) Whole mount in situ hybridization analysis showing the expression of raldh4 mRNA. (B-C,E,H) Lateral view, anterior left, dorsal top; (D,F) dorsal view, anterior left; (B) 2 dpf; (C-D) 3 dpf; (E,F) 5 dpf; (H) 7 dpf; (G) sagittal section of 5 dpf embryos through intestinal bulb; (I) transverse section of 7 dpf embryos through intestinal bulb. li, liver; ib, intestinal bulb.
Reprinted from Gene expression patterns : GEP, 8(4), Liang, D., Zhang, M., Bao, J., Zhang, L., Xu, X., Gao, X., and Zhao, Q, Expressions of Raldh3 and Raldh4 during zebrafish early development, 248-253, Copyright (2008) with permission from Elsevier. Full text @ Gene Expr. Patterns