Fig. S2

Fig. S2 Tjp3/Zo-3 is essential for EVL permeability barrier function. A. Biotin permeability assay: control morpholino (Cmo) and tjp3/zo-3 translation blocking morpholino (ATGmo) injected embryos were dechorionized at 6 hpf and exposed to 1 mg/ml Biotin in egg water. Cryosections were probed with labeled streptavidin and images taken using identical parameters. In Cmo injected embryos, although some background staining for streptavidin was observed, only little biotin diffusion was detected. In 6 out of 9 ATGmo injected embryos (correlating with the penetration rate of the morphant phenotpye), a significant accumulation of biotin was observed, in particular in the segmentation cavity between the blastoderm and the yolk syncythial layer, demonstrating a compromised epidermal barrier in embryos lacking Tjp3/Zo-3.